Knock-down of three core transcription factors in hEC cells
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资源简介：
Three transcription factors were silenced in human embryonic carcinoma cells, OCT4, NANOG, and SOX2. Downstream effects were analysed by means of microarrays to obtain insights into the regulation of self-renewal in these cells. Keywords: RNAi-chip Cells were transfected with esiRNA against the respective transcription factor transcripts. Knock-down efficiencies were high as monitored by real-time RT-PCR and Western blotting. Samples for chip hybridisations were harvested 2.5 days after transfection.
创建时间：
2012-03-16
