Ip, Wallace (2010) CIL:6258, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were blended in the presence of 5 mM MgATP to dissociate thick and thin filaments. The medium was then gently replaced with 0.3M KCl to dissociate thick filaments. Dissociated filaments were adsorbed onto mica flakes (Heuser JE, J Mol Biol. 1983 Sep 5;169(1):155-95), quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

This image was recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:6267.

兔子的腰大肌骨骼肌纤维在5 mM MgATP的存在下进行混合，以分离粗肌丝和细肌丝。随后，以0.3M KCl温和替换介质，以进一步分离粗肌丝。分离的肌丝被吸附在云母片（Heuser JE, J Mol Biol. 1983 Sep 5;169(1):155-95）上，并通过与液氦冷却的铜块接触在Heuser型冷冻压机中快速冷冻，并在Balzers 400冷冻断裂机中进行冷冻蚀刻。样品随后在11度角下进行铂-碳旋转复制，并在运行于100 kev的JEOL 100CX2透射电子显微镜下进行可视化观察。图像记录于柯达4489胶片上，放大倍数为75,000倍。在+5度和-5度处拍摄了立体对图像。胶片使用尼康Coolscan 9000ED扫描仪以20微米像素间距进行扫描。与CIL:6267的立体对图像相匹配。