Genome-wide analysis of Wild Type and Relb-/- MEFs

Recent studies indicated that the NF-kB RelB subunit may play roles beyond immune modulations. The molecular mechanism that may allow RelB to engage in such diverse physiological activities remains unclear. To better understand the RelB-driven transcriptome, therefore, we subjected wild type and Relb-/- mouse embryonic fibroblast (MEF) to microarray analyses using Illumina MouseRef-8 v2.0 Expression BeadChip. We propose that our transcroptomic analyses will help linking RelB to other important cellular pathways. Total RNA was isolated from mouse embryonic fibroblast(MEFs) of Wild Type and Relb-/- genetic background and microarray was performed.