Detection of circulating arboviruses from mosquito homogenates by 3G1 and 2G4.

Legend: + positive;—negative.
* Viruses previously identified using virus specific monoclonal antibodies in ELISA
# Fixed-cell ELISA using mAbs 3G1 and 2G4 against four wells of C6/36 cells inoculated with mosquito homogenate. Samples were deemed positive if at least one well gave strong positive in ELISA.
&RT-PCR using flavivirus- and alphavirus-specific primers was performed on RNA extracted from supernatant harvested from inoculated wells prior to fixing.
1CPE was observed in these samples upon subsequent passaging during our analysis.
Negative, no virus was identified by reagents specific for known viruses; SINV, Sindbis virus; WNVKUNV, West Nile virus subtype Kunjin; ALFV, Alfuy virus; KOKV, Kokobera virus.
Detection of circulating arboviruses from mosquito homogenates by 3G1 and 2G4.