Transcription profiling by high throughput sequencing of Sox17.Epi and Endo cells from mouse embryos

This experiment has been done to understand the dynamics of gene expression in Sox17.Epi and Endo cells using multiplex RNA-Seq technology. Sox17.Epi (Sox17^GFP+EpCAM+) and Sox17.Endo (Sox17^GFP+EpCAM-) cells were isolated by FACS from E9.5 mouse embryos. The spatial comparison between Sox17.Epi versus Sox17.Endo provides insight into which genes are differentially of specifically expressed in two populations.本实验旨在借助多重RNA测序（multiplex RNA-Seq）技术，解析Sox17.Epi细胞与Sox17.Endo细胞的基因表达动态变化规律。其中，Sox17.Epi细胞（Sox17^GFP+EpCAM+）与Sox17.Endo细胞（Sox17^GFP+EpCAM-）均通过荧光激活细胞分选（FACS）技术从E9.5期小鼠胚胎中分离获取。对两类细胞群体开展空间比对分析，可明确在二者中差异表达或特异性表达的基因，从而为相关研究提供理论洞察。