Effects of sdo1 gene deletion on cell division of fission yeast at and its molecular mechanism

The sdo1 gene encodes SBDS family ribosome assembly protein, which is involved in the ribosome biosynthesis. However, it is not yet known whether it is involved in the regulation of cell mitosis dynamics. This study investigated the impact of sdo1 gene deletion on cell mitosis dynamics in fission yeast (Schizosaccharomyces pombe) by live cell imaging and bio-sequencing analysis. The results showed that sdo1 deletion contributed to slow cell growth and abnormal spore production. RNA-Seq analysis indicated that rex3 up-regulation and mfm1/mfm2 down-regulation were key factors contributing to the increased spore production. nog1 up-regulation in the ribosome biogenesis pathway and nad3 down-regulation were associated with increased spindle elongation, and eng1 and chs2 down-regulation extended the time from septa formation to separation, while act1 down-regulation prolonged the length of the actin ring. This work reveals the effect of sdo1 gene deletion on cell division at different temperatures, which provides some scientific basis for exploring the function of Sdo1 protein in cell division.