Fibrosis-memory is mediated by IL-3-producing T cells and drives progression of fibrosis

Repetitive injuries are an important trigger of progressive fibrosis. To study if repetitive injuries induce an accelerated pro-fibrotic process, also called “fibrosis-memory”, we established an experimental system with two consecutive, clearly separated insults in a model of renal fibrosis with reversible ureteral obstruction of the right kidney (from day 0-6) and irreversible unilateral ureteral obstruction of the left kidney (from day 17-22). Overall design: 5' end sequencing of Tcrb mRNAs in T cells from spleen and left UUO-kidney on day 22