Single cell-seq identify discoid lateral meniscus

Discoid lateral meniscus (DLM) is more prone to injury than a normally shaped meniscus. Our previous study successfully identified the different cell types and their corresponding marker genes in meniscus tissues, but there was no study comparing the gene expression and cell heterogenicity of discoid meniscus with normal meniscus. Overall design: scRNA-seq was used to identify cell subsets and their gene signatures in DLM and osteoarthritis lateral meniscus (OAM) cells to determine their differentiation relationships and identify specific cell types. Pseudotime analysis and immunohistochemical staining were used to investigate temporal and spatial distribution of DLM specific clusters