In the presence of pre-formed mast cell mediators, SVEC4-10 cells form tubes and loops.

The cells were cultured for 5 hours at 37°C on Geltrex® in the presence of Mast Cell Mediators (MCM) from bone marrow derived mast cell. (A) When the SVEC4-10 cells were cultured in the presence of MCM, most of the cells were spread on the substrate, and formed both tubes (T) and loops (L). (B) When the cells were incubated in absence of mast cell mediators, the cells were spread on the substrate forming tubes (T), but there was no loop formation. (C) Quantitative analysis shown that the tube formation was higher in presence of mast cell mediators. The significance was determined by Student's t-test *p ≤ 0.05.