Monolayer neural differentiation of individual gene trap clones.

Monolayer neural differentiation of twenty clones from gene trap mutation pool 1 is presented. Clones with Tcf3 mutation are labelled with “*”. Two clones from mutant pool 2 were also included as a control for monolayer differentiation assay. “D” represents clones showing extensive neural differentiation. “Non-D” represents cells showing predominantly undifferentiated ES cell morphology. P1-8 cells differentiated to flat non-neural cells.