Coordinated metabolic transitions and gene expression by NAD+ during adipogenesis.

Adipocytes are the main cell type in adipose tissue, a critical regulator of metabolism, highly specialized in storing energy as fat. Adipocytes differentiate from multipotent mesenchymal stromal cells through adipogenesis. Using RNA-seq we explored the effect of manipulating NAD+ bioavailability during adipogenic differentiation from human mesenchymal stem cells. Cells were harvested either undifferentiated (hMSC) or after days 8 and 16 of adipogenic induction. During adipogenesis, cells were treated with either NAD+ (5 mM), or FK866 (1 nM) or EX527 (50 µM). Overall design: mRNA was obtained from human mesenchymal stem cells (hMSC) either undifferentiatiated or at days 8 and 16 after adipogenic induction. During differentation, cells were trated with NAD+ (5 mM), FK866 (1 nM) or EX527 (50 µM). Each condition was analyzed per triplicate.