Integrated multi-omics reveals putative biomarkers of bacterial-fungal co-infections

Transcriptomic analysis of Aspergillus fumigatus and Klebsiella pneumoniae grown as single and mixed biofilms. RNA Sequencing was performed of Aspergillus fumigatus and Klebsiella pneumoniae grown as single and mixed biofilms for 24 hours at 37 °C in YPD medium. For mixed biofilms, Aspergillus fumigatus was pre-cultured for 12 hours to allow germination prior addition of Klebsiella pneumoniae. Cultures were harvested and three biological replicates were subjected for RNA isolation and sequencing library preparation.