Enspyre Proof-of-Concept

Hybridization capture has been a mainstay of molecular enrichment technologies over the last 15 years, providing robust enrichment of target molecules. However, it typically requires significant sequencing depth to capture rare variants, which limits its efficiency and increases costs, especially in applications like minimal residual disease (MRD) monitoring and early cancer detection. Enspyre (Enrichment by selective pyrophosphorolysis and release) is a novel technology designed to address these limitations by enabling selective enrichment of specific variants prior to sequencing. The dataset presented here consists of NGS sequencing data from the Enspyre MRD proof-of-concept experiments, which comprise serial dilutions of contrived reference material (Genome in a Bottle).