Effects of the interactive sequences of αB crystallin on microtubule assembly.

Column one lists the bioactive peptides based on the interactive sequences in αB crystallin and tested previously on microtubule assembly [15]. Column two lists the corresponding residues in human αB crystallin. Column three lists the location in human αB crystallin of each interactive sequence. Column four lists the effect of each peptide on microtubule assembly; “NE” indicates “no effect”, “−” indicates inhibition and “+” indicates promotion of assembly. Column five lists the involvement of each sequence in subunit-subunit interactions. The two peptides that promote microtubule assembly are sequences for subunit-subunit interactions and the peptide that inhibits microtubule assembly is not. The interactions between tubulin and αB crystallin occur at sites where these sequences are exposed on the surface of the αB crystallin subunit.