PIF7 DNA binding in response to long days with daily oscillating temperatures in Arabidopsis thaliana

This study aimed to characterise the genome-wide PIF7-mediated responses of Arabidopsis thaliana seedlings to long photoperiods with a warm 27°C midday in comparison to long photoperiods at constant 17°C. Transcriptomic responses were observed in Col-0 wild-type and pif7-1 loss-of-function mutants investigate the potential contribution of the transcription factor PIF7 to the warm temperature response under the described conditions. In parallel, ChIP-seq of a PIF7-MYC-expressing line under the same conditions was employed to investigate global DNA binding changes of PIF7 in response to a change in temperature. Overall design: Single experiments at different growth conditions. Sample 186CS12 has been included for its superior efficiency while 192C series shows better differential binding.