Single cell transcriptome of the mouse larynx

Microbiota play a critical role in training and development of major components of host innate and adaptive immune system. We present the cellular landscape of the upper airway, specifically the larynx, by establishing a reference single-cell atlas and dissected the role of microbiota in laryngeal cell development and functions at a single-cell resolution by comparing the transcriptomes of conventionally raised and germ-free mouse larynges. Overall design: Single cell suspension of the mouse larynx was prepared for conventionally raised and germ-free mice and sequenced and analyzed following 10x genomics pipeline.