Supplemental figures for: FtsK is critical for the assembly of the unique divisome complex of the FtsZ-less Chlamydia trachomatis IF images

# Supplemental figures for: FtsK is critical for the assembly of the
unique divisome complex of the FtsZ-less Chlamydia trachomatis IF images
[https://doi.org/10.5061/dryad.2bvq83c27](https://doi.org/10.5061/dryad.2bvq83c27) ## Description of the data and file structure All .zvi files can be viewed using Fiji or Zeiss Zen Blue software, which is available as a free download. Arrows depict cells of interest. Stages of division were determined by the distribution of the major outer membrane (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera.   ### Files and variables #### File: figure2\_figuresupplement1C\_mcherry\_pbp2multipbud.zvi **Description:** Figure 2-Figure Supplement 1C-Source Data 1. Original .zvi file showing mCherry-PBP2 (red - mCherry fluorescence) localization to the septum and base in a cell containing a secondary bud site at the base at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement1C\_mcherry\_pbp3multipbud.zvi **Description:** Figure 2-Figure Supplement 1C-Source Data 2. Original .zvi file showing mCherry-PBP3 (red - mCherry fluorescence) localization to the septum and base in a cell containing a secondary bud site at the base at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement1C\_mreb\_6xhismultipbud.zvi **Description:** Figure 2-Figure Supplement 1C-Source Data 3. Original .zvi file showing MreB_6xHis (red fluorescence) localization to the septum and base in a cell containing a secondary bud site at the base at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement1C\_mcherry\_mrecmultipbud.zvi **Description:** Figure 2-Figure Supplement 1C-Source Data 1. Original .zvi file showing mCherry-MreC (red - mCherry fluorescence) localization to the septum and base in a cell containing a secondary bud site at the base at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: Figure2\_figure\_supplement\_2B\_mcherry\_pbp2\_pbp2antibody.zvi **Description:** Figure 2-Figure Supplement 2B-Source Data 1. Original .zvi file showing endogenous PBP2 (blue fluorescence) and mCherry-PBP2 (red-mCherry fluorescence) localization to the septum in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera.   #### File: Figure2\_figure\_supplement\_2B\_mcherry\_pbp3\_pbp3antibody.zvi **Description:** Figure 2-Figure Supplement 2B-Source Data 2. Original .zvi file showing endogenous PBP3 (blue fluorescence) and mCherry-PBP3 (red-mCherry fluorescence) localization to the septum in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera.   #### #### File: figure2\_figuresupplement3A\_endogenous\_pbp2\_coccoid.zvi **Description:** Figure 2-Figure Supplement 3A-Source Data 1. Original .zvi file showing endogenous PBP2 (red fluorescence) localization in a coccoid cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement3A\_endogenous\_pbp3\_coccoid.zvi **Description:** Figure 2-Figure Supplement 3A-Source Data 2. Original .zvi file showing endogenous PBP3 (red fluorescence) localization in a coccoid cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement3A\_endogenous\_pbp2\_septum.zvi **Description:** Figure 2-Figure Supplement 3A-Source Data 3. Original .zvi file showing endogenous PBP2 (red fluorescence) localization to the septum in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement3A\_endogenous\_pbp3\_septum.zvi **Description:** Figure 2-Figure Supplement 3A-Source Data 4. Original .zvi file showing endogenous PBP3 (red fluorescence) localization to the septum in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement3A\_endogenous\_pbp2\_septum\_and\_base.zvi **Description:** Figure 2-Figure Supplement 3A-Source Data 5. Original .zvi file showing endogenous PBP2 (red fluorescence) localization to the septum and base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement3A\_endogenous\_pbp3\_septum\_and\_base.zvi **Description:** Figure 2-Figure Supplement 3A-Source Data 6. Original .zvi file showing endogenous PBP3 (red fluorescence) localization to the septum and base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement3A\_endogenous\_pbp2\_base.zvi **Description:** Figure 2-Figure Supplement 3A-Source Data 8. Original .zvi file showing endogenous PBP2 (red fluorescence) localization to the base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement3A\_endogenous\_pbp3\_base.zvi **Description:** Figure 2-Figure Supplement 3A-Source Data 8. Original .zvi file showing endogenous PBP3 (red fluorescence) localization to the base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement3A\_endogenous\_pbp2\_secondarybud.zvi **Description:** Figure 2-Figure Supplement 3A-Source Data 9. Original .zvi file showing endogenous PBP2 (red fluorescence) localization to the septum and base in a cell containing a secondary bud site at the base at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement3A\_endogenous\_pbp3\_secondarybud.zvi **Description:** Figure 2-Figure Supplement 3A-Source Data 10. Original .zvi file showing endogenous PBP3 (red fluorescence) localization to the septum and base in a cell containing a secondary bud site at the base at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement4A\_endogenous\_FtsK\_mCherryPBP2\_septum\_and\_base.zvi **Description:** Figure 2-Figure Supplement 4A-Source Data 1. Original .zvi file showing endogenous FtsK (blue fluorescence) and mCherry-PBP2 (red-mCherry fluorescence) localization to the septum and base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement4A\_endogenous\_FtsK\_mCherryPBP2\_base.zvi **Description:** Figure 2-Figure Supplement 4A-Source Data 2. Original .zvi file showing endogenous FtsK (blue fluorescence) and mCherry-PBP2 (red-mCherry fluorescence) l localization to the base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement4A\_endogenous\_FtsK\_mCherryPBP3\_septum\_and\_base.zvi **Description:** Figure 2-Figure Supplement 4A-Source Data 3. Original .zvi file showing endogenous FtsK (blue fluorescence) and mCherry-PBP3 (red-mCherry fluorescence) localization to the septum and base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement4A\_endogenous\_FtsK\_mCherryPBP3\_base.zvi **Description:** Figure 2-Figure Supplement 4A-Source Data 4. Original .zvi file showing endogenous FtsK (blue fluorescence) and mCherry-PBP3 (red-mCherry fluorescence) localization to the base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement4A\_endogenous\_FtsK\_MreB\_6xHis\_septum\_and\_base.zvi **Description:** Figure 2-Figure Supplement 4A-Source Data 5. Original .zvi file showing endogenous FtsK (blue fluorescence) and MreB_6xHis(red fluorescence) localization to the septum and base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement4A\_endogenous\_FtsK\_MreB\_6xHis\_base.zvi \**Description: **Figure 2-Figure Supplement 4A-Source Data 6. Original .zvi file showing endogenous FtsK (blue fluorescence) and MreB_6xHis(red fluorescence) localization to the base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement4A\_endogenous\_FtsK\_mCherryMreC\_septum\_and\_base.zvi **Description:** Figure 2-Figure Supplement 4A-Source Data 7. Original .zvi file showing endogenous FtsK (blue fluorescence) and mCherry-MreC (red-mCherry fluorescence) localization to the septum and base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: figure2\_figuresupplement4A\_endogenous\_FtsK\_mCherryMreC\_base.zvi **Description:** Figure 2-Figure Supplement 4A-Source Data 8. Original .zvi file showing endogenous FtsK (blue fluorescence) and mCherry-MreC (red-mCherry fluorescence) localization to the base in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: Figure\_2\_figure\_supplement5A\_mCherry\_MreC\_ring\_dividing\_cell.zvi **Description:** Figure 2-Figure Supplement 4A-Source Data 1. Original .zvi file showing mCherry-MreC (red-mCherry fluorescence) localization at the septum in a dividing cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. #### File: Figure\_2\_figure\_supplement5B\_mCherry\_MreC\_ring\_coccoid\_cell.zvi **Description:** Figure 2-Figure Supplement 4B-Source Data 1. Original .zvi file showing mCherry-MreC (red-mCherry fluorescence) localization in a coccoid cell at 21 hpi. Stages of division were determined by the distribution of the major outer membrane protein (green fluorescence). Cells were imaged using a Zeiss AxioImager2 microscope equipped with a 100x oil immersion PlanApochromat objective and a CCD camera. ## Code/software All .zvi and .czvi files can be viewed using Fiji software or Zeiss Zen Blue software, which is available as a free download. Arrows depict cells of interest.  ## Access information NA