Real-time quantitative PCR analysis of human plasma miRNA from rheumatoid arthritis patients with or without interstitial lung disease

RNA was isolated from 200μl plasma samples and cDNA was synthesized. Real-time RT-PCR analysis was performed to evaluate miRNA expression in the plasma pool from 17 RA patients with RA-ILD or the plasma pool from 17 RA patients without ILD using Human miRNome microRNA PCR Panel I+II (Exiqon). qPCR miRNA expression profiling. Plasma samples from 17 donors were used in control and test.