Small Noncoding RNAome Changes during macrophage polarization

Macrophages belong to a special phagocytic subgroup of human leukocytes and are one of the important cells of the human immune system. They participate in both specific and innate immune processes and play an important role in the host's resistance to pathogen invasion and the inflammatory response. Small noncoding RNAs are a group of small RNA molecules that can be transcribed without the ability to encode proteins but could play a specific function in cells. We used high-throughput sequencing analysis and qPCR to detect the expression changes of the small noncoding RNAome during macrophage polarization. Overall design: We used RAW264.7 cells to induce macrophage polarization. For M1 macrophage polarization, we added LPS and IFN-? stimulated RAW264.7 cells. For macrophage M2 polarization, we stimulated RAW264.7 cells with IL-4 and IL-10.