Reduced Abd-B Hox function during kidney development results in lineage infidelity

In this study, in order to better understand shared core Hox functions, we examined kidney development in mice with frameshift mutations of multiple Abd-B type Hox genes. The resulting phenotypes included dramatically reduced branching morphogenesis of the uretreric bud, premature depletion of nephron progenitors and abnormal development of the stromal compartment. Most unexpected, however, we also observed a cellular level lineage infidelity in nephron segments. E14.5 mouse kidney cells were analyzed using Drop-seq本研究为深入解析共享的核心Hox基因功能，我们对携带多个Abd-B型Hox基因移码突变的小鼠肾脏发育进程进行了系统考察。所获得的表型包括输尿管芽分支形态发生显著受损、肾单位祖细胞过早耗竭，以及间质室发育异常。然而最为意外的是，我们还在肾单位节段中观测到了细胞水平的谱系保真缺陷。本研究采用Drop-seq技术对E14.5胎龄的小鼠肾脏细胞开展了分析。