The insect midgut is not restful during the larva-to-larva molting stage

IMolting, a special period during which the old cuticle is shed and a new one is produced, is crucial to insect development. During their life cycles, insects that undergo complete metamorphosis may experience several larva-to-larva moltings to become larger, followed by larva-to-pupa and pupa-to-adult moltings to become adults. During the larva-to-larva molting stage, insect larvae stop consuming food and become restful. Whether any changes occur within the molting midgut before ecdysis remains known. Total RNA from day 3 of 4th feeding, 4th molting and 5th feeding stages Bombyx midgut were used to generate target cDNA, and then hybridized to 48k Bombyx genome Array Genechips, representing about 23000 characterized genes