Tape Strip Profiling of Checkpoint Inhibitor-Associated Dermatitis Highlights Pan-T-Cell Activation: A Pilot Study

This pilot study examined gene expression in lesional and non-lesional tape strip samples obtained from 5 patients with immune-checkpoint inhibitor (ICI) eczematous reactions/ICI-Ecz, 3 patients with ICI lichen planus-type reactions/ICI-LP, and 8 patients with atopic dermatitis (AD), as well as from 9 demographically matched healthy controls. Differential expression was defined with |fold-change/FCH|>2 and false discovery rate/FDR<0.1. Significant upregulation in Th1 and Th2, notably in IL4R, was observed in both ICI groups. JAK/STAT upregulation was additionally observed in ICI-Ecz and AD. Overall design: RNA-seq was performed on lesional and non-lesional tape strip samples obtained from 5 patients with immune-checkpoint inhibitor (ICI) eczematous reactions/ICI-Ecz, 3 patients with ICI lichen planus-type reactions/ICI-LP, and 8 patients with atopic dermatitis, as well as from 9 demographically matched healthy controls. Differential expression was defined with |fold-change/FCH|>2 and false discovery rate/FDR<0.1.