Oncogenic Antisense LncRNA P14AS Regulates Expression of ANRIL through AUF1 Binding

The CDKN2A/B locus at 9p21.3 contains crucial tumor suppressor (P16, P14, and P15) and oncogenic lncRNA ANRIL genes. This locus is most frequently inactivated in cancer genomes by deletion and DNA methylation. However, the mechanisms coordinately regulating their expression level are far from clear. In the present study, a novel lncRNA, P14AS, was characterized in the antisense strand of the fragment near CDKN2A exon1? in human cell lines using CDKN2A-specific probe captured RNA-sequencing (RNACap-Seq). Overall design: Different gene expression in HCT116 cells with P14AS stable over-expression and/or transient AUF1 knockdown at 72 post-transfection hrs.