Molten-globule conformation of Arc repressor monomers determined by high-pressure 1H NMR spectroscopy.
收藏PubMed Central1993-03-01 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC45962/
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资源简介：
The conformation of the pressure-dissociated monomer of Arc repressor was characterized by 1H NMR spectroscopy. The NMR spectra of the monomer under pressure (up to 5.0 kbar; 1 bar = 100 kPa) are typical of a molten globule and they are considerably different from those of the native dimer and thermally denatured monomer. The two-dimensional nuclear Overhauser effect spectra suggest that the pressure-induced molten globule retains some secondary structure. The presence of nuclear Overhauser effects in the beta-sheet region in the dissociated state suggests that the intermonomer beta-sheet (residues 8-14) in the native dimer is replaced by an intramonomer beta-sheet. Changes in one-dimensional and two-dimensional NMR spectra prior to pressure dissociation were found and suggest the existence of a "predissociated" state. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1993-03-01
