The function and mechanisms of hsa_circCDKN2B-AS_012 on tumor suppression of Colorectal cancer

Circular RNAs (circRNAs) are a class of non-coding RNAs with a covalently closed loop structure, which plays crucial roles in regulating tumor development and progression. However, the detailed molecular mechanism of circANRIL in CRC is not fully understood. Real-time quantitative PCR was used to examine the expression of circ86591 in cells. RNA pull-down, mass spectrometry, RNA immunoprecipitation and western blotting were applied to investigate the possible molecule mechanisms of circ86591 and its binding proteins. RNA-sequencing, gain-of-function and loss-of function experiments were performed to find tumor-suppressive effect and the relevant signaling pathways of circ86591. Overall design: To investigate the relevant signaling pathways of circ86591 in the regulation of cell proliferation, we established LOVO cells transfected with ASO-nc or ASO-circ86591 and HCT116 cells infected with ADV ctrl and ADV circ86591.