Data from: Global change in brain state during spontaneous and forced walk in Drosophila is composed of combined activity patterns of different neuron classes

Movement-correlated brain activity has been found across species and brain
regions. Here, we used fast whole-brain lightfield imaging in adult
Drosophila to investigate the relationship between walk and brain-wide
neuronal activity. We observed a global change in activity that tightly
correlated with spontaneous bouts of walk. While imaging specific sets of
excitatory, inhibitory, and neuromodulatory neurons highlighted their
joint contribution, spatial heterogeneity in walk- and turning-induced
activity allowed parsing unique responses from subregions and sometimes
individual candidate neurons. For example, previously uncharacterized
serotonergic neurons were inhibited during walk. While activity onset in
some areas preceded walk onset exclusively in spontaneously walking
animals, spontaneous and forced walk elicited similar activity in most
brain regions. These data suggest a major contribution of walk and
walk-related sensory or proprioceptive information to global activity of
all major neuronal classes.