Gene expression analysis on the immortalized human mesothelial cell lines with different morphologies.. Gene expression analysis on the immortalized human mesothelial cell lines with different morphologies.

Establishment of immortalized human omental mesothelial cell lines (HOMCs) resulted in cloning three sublines (HOMC-B1, A4 and D4) with different morphologies. HOMC-B1 forms an epithelial-like monolayer. HOMC-A4 shows spindle-shaped morphology (storiform pattern). HOMC-D4 displayed an intermediate appearance. With the aim of exploring the key genes on decision of cellular morphology, gene expression of HOMC cell lines were analyzed along with conventional normal pleura-derived cell line, MeT-5A. Overall design: Array analysis was performed with three HOMC cell lines with different morphology and human normal pleura transformant MeT-5A as reference cells.