锌离子高度增强固定化β-葡萄糖苷酶的耐热性研究
收藏国家林业和草原科学数据中心2022-11-14 更新2024-03-06 收录
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The immobilization of thermostable GH3 β-glycosidase (Tpebgl3) was obtained by using NKA-9 macroporous resin modified with PEI and glutaraldehyde. The properties of NKA-9II, including thermal stability, pH stability, reusability, and tolerance of glucose, were greatly improved compared with free Tpebgl3. Moreover, this manuscript provides the first report that the coordinating metal cation Zn2+ enabled an immobilized enzyme to exhibit higher catalytic efficiency. Meanwhile, the addition of Zn2+ could greatly advance the reusability, thermostability, and glucose tolerance of NKA-9II compared with that with free metal ion, which is extremely beneficial for industrial applications.
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国家林业和草原科学数据中心
创建时间:
2022-11-14



