Gene expression signatures in oridonin-treated WT and R878H hematopoietic stem cells

Analysis of WT-Ctl v.s. WT-Ori and R878H-Ctl v.s. R878H-Ori hematopoietic stem cell (lineage-, c-kit+ Sca-1+ CD150+, CD34-). The four population of hematopoietic stem cell (HSC) were purified from the bone marrow of WT and R878H recipent mice treated with vehicle or oridonin. Results provide insight into the role of oridonin in R878H HSC. WT-Ctl, WT-Ori, R878H-Ctl and R878H-Ori HSCs from the bone marrow of recipient mice, then subjected into deep sequencing, in triplicate, using Illumina HiSeq platform.