Transcription profiling by array of mouse lung from C57BL/6J and A/J mice during embryonic development

In this study, time dependent genome wide lung mRNA profiling changes were assessed using C57BL/6J and A/J mice. Through comprehensive bioinformatics and functional genomics analyses, we identified both temporal and strain dependent gene expression patterns, systemically mapped key regulators, bioprocesses, and transcriptional networks controlling lung maturation, providing the basis for new therapeutic strategies to enhance lung function in preterm infants. In this study, we designed a genome-wide mRNA expression time-course study from E15.5 to Postnatal Day 0 (PND0) using lung RNAs from C57BL/6J and A/J mice that differ significantly in gestational length.本研究以C57BL/6J与A/J小鼠为实验对象，评估了肺组织全基因组信使RNA（mRNA）表达谱的时间依赖性变化。通过综合生物信息学与功能基因组学分析，本研究鉴定出兼具时间与品系特异性的基因表达模式，系统绘制了调控肺成熟的关键调控因子、生物过程及转录网络，为改善早产儿肺功能的新型治疗策略提供了理论依据。 本研究针对妊娠时长存在显著差异的C57BL/6J与A/J小鼠，采集其肺组织RNA，开展了从胚胎15.5天（E15.5）至出生后第0天（PND0）的全基因组mRNA表达时序研究。