Summary of adult lifespan assays after RNAi-mediated gene inactivation.

RNAi treatments, initiated at L1 stage, were continued throughout the assay time. Lifespan assays were performed at 20°C. Each experiment was individually grouped and statistical analyses of the data sets were carried out using the vector (L4440) control as the reference in each group (P value). In Set #3, the second set of statistical analyses was carried out for all the survivor data derived from the asm-3(ok1744);rrf-3(pk1426) strain, using the corresponding vector (L4440) control in this strain (marked as *) as the reference for this subgroup (*P values). Each set of the lifespan experiments was repeated at least two independent times and similar results were obtained. Data from representative sets of experiments are shown. Greater than 50 worms were counted for each RNAi-inducing condition in each experiment.