Transcriptional profiling of OT-1 cells during in vitro memory formation after high- or low-affinity priming. Mus musculus

To understand the impact of TCR affinity during priming on memory formation, we performed transcriptional profiling of OT-1 cells at different time points after stimulation with high- or low-affinity ligands. Our findings indicate that low-affinity stimulaation is associated with earlier memory formation. Overall design: We stimulated purified OT-1 T cells with high-affinity N4 or low-affinity Q4 peptides, icw anti-CD28. After 30h cells were washed and cultured with IL-15 or IL-2 for an additional 5 days. RNA was isolated at T0, T30, T72 and T140.