Changes in the status of spermatogenesis in the rat testis following a knockdown of Rai14 by RNAi*.

*Approximately 300 seminiferous tubules at specified stages from each rat testis transfected with Rai14 specific siRNA duplexes (Rai14 RNAi) vs.testis transfected with non-targeting control siRNA duplexes (Ctrl RNAi) were randomly selected and scored to assess defects in spermatogenesis, and a total of 3 rats from each group were scored. Data were expressed as percentage of tubules at annotated stages having defects in Rai14 RNAi vs. Ctrl RNAi rats.**Tubules at stage VI-early VIII that contained more than 5 spermatids, displaying a defect in polarity in the seminiferous epithelium in which spermatid heads no longer pointing toward the basement membrane but at least 90° deviated from their normal orientation, were scored as a defect in spermatid polarity.***Tubules at stage IX-X that contained more than 5 elongating/elongated spermatids that were trapped in the seminiferous epithelium and failed to undergo spermiation at stage VIII were counted as a defect in spermiation.