Table1_Brain and Pituitary Transcriptome Analyses Reveal the Differential Regulation of Reproduction-Related LncRNAs and mRNAs in Cynoglossus semilaevis.DOCX

Long noncoding RNAs (lncRNAs) have been identified to be involved in half-smooth tongue sole (Cynoglossus semilaevis) reproduction. However, studies of their roles in reproduction have focused mainly on the ovary, and their expression patterns and potential roles in the brain and pituitary are unclear. Thus, to explore the mRNAs and lncRNAs that are closely associated with reproduction in the brain and pituitary, we collected tongue sole brain and pituitary tissues at three stages for RNA sequencing (RNA-seq), the 5,135 and 5,630 differentially expressed (DE) mRNAs and 378 and 532 DE lncRNAs were identified in the brain and pituitary, respectively. The RNA-seq results were verified by RT-qPCR. Moreover, enrichment analyses were performed to analyze the functions of DE mRNAs and lncRNAs. Interestingly, their involvement in pathways related to metabolism, signal transduction and endocrine signaling was revealed. LncRNA-target gene interaction networks were constructed based on antisense, cis and trans regulatory mechanisms. Moreover, we constructed competing endogenous RNA (ceRNA) networks. In summary, this study provides mRNA and lncRNA expression profiles in the brain and pituitary to understand the molecular mechanisms regulating tongue sole reproduction.

长非编码RNA（lncRNA）已被确认为与半平滑舌鳐（Cynoglossus semilaevis）的繁殖过程密切相关。然而，关于它们在繁殖过程中所扮演角色的研究主要集中于卵巢，其在脑部和垂体中的表达模式及其潜在作用尚不明确。因此，为探究与脑部和垂体繁殖密切相关的mRNA和lncRNA，本研究收集了不同发育阶段的舌鳐脑部和垂体组织，进行RNA测序（RNA-seq），分别鉴定出5,135和5,630个差异表达（DE）的mRNA以及378和532个差异表达的lncRNA。RNA-seq结果经实时定量PCR验证。此外，还进行了富集分析，以解析差异表达mRNA和lncRNA的功能。有趣的是，它们在代谢、信号传导和内分泌信号通路中的参与作用得以揭示。基于反义、顺式和反式调控机制，构建了lncRNA靶基因相互作用网络。此外，还构建了竞争性内源RNA（ceRNA）网络。总之，本研究提供了脑部和垂体中mRNA和lncRNA的表达谱，旨在揭示调控舌鳐繁殖过程的分子机制。