Dendritic cells DAGL-beta, Vaccination

The purpose of this experiment was to test whether the DAGL-beta inhibition in dendritic cells affects DC priming ability to T-cells.
Conclusion:
There were no differences in DAGL-beta or alpha disruption compared to wild type dendritic cells.
Notes:
Dendritic cells were activated and injected into Black 6 mice. After 7 days, spleens from the mice were harvested and splenocytes were analyzed for CD8T cells that are expressing the labeling with OVA-dex Quality controls were performed by testing the experimental conditions against the dendritic cells from wild type, DAGL-beta KO, and DAGL-alpha KO mice. The same number of cells were injected and mice were allowed to remain at the same vivarium with identical conditions. Cells resulting from the analyses were subjected to the same gating strategy for quality control.