SEC analysis of concentrations of species.
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Concentrations of the five species in scheme 1 determined from SEC measurements as described in the text using different initial concentrations (1∶1:1 of Atox1:Cu:WD4) as indicated. Also, the % of the total copper found in hetero-complex is reported. The equilibrium concentrations established are used to derive K1 and K2 and from this the copper exchange factor K1*K2 is calculated. Two experiments with 150 µM starting concentrations are reported. For 75 µM, also the opposite reaction, mixing Cu-WD4 with apo-Atox1 was performed.
创建时间：
2015-12-02
