HuD interacting circular RNAs in the mouse striatum
收藏NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE153405
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资源简介：
This study explores whether HuD could bind to and regulate the expression circRNAs from genes associated with neuronal development and synaptic plasticity circRNAs bound to HuD were isolated from the striatum of HuD-OE mice by RNA immunoprecipitation (RIP) as described in Bolognani et al, 2010 (https://doi.org/10.1093/nar/gkp863) using Dynabeads® (Thermo Fisher Scientific) coated with mouse monoclonal anti-myc tag antibody (9B11; Cell Signalling Technology Inc.) specifically recognizing myc-tagged HuD transgenic protein expressed in HuD-OE as described before (Bolognani et al., 2010; Zimmerman et al., 2020). Controls for RIP assays were performed using either non-immune IgG and HuD-OE tissue or the myc-tag antibody and wild type (WT) tissue. RIP assay were run in triplicates using 3 HuD transgenic and 3 wildtype mice
创建时间：
2020-08-31
