the bone marrow MicroRNA of beagle dogs Raw sequence reads

Toxocara canis is distributed worldwide, posing a serious threat to both human and dogs health, however, the mechanism of T. canis infection remains unclear. In the present study, the changes of miRNA expression profiles in bone marrow of Beagle dogs were investigated by RNA-seq and bioinformatics analysis. A total of 39 differentially expressed (DE) miRNAs were identified in this study. Among which, 4 DEmiRNAs were identified at 24 post-infection (hpi), all of which were up-regulated; 8 DEmiRNAs were identified with 2 up-regulated miRNAs and 6 down-regulated miRNAs at 96 hpi; 27 DEmiRNAs were identified with 13 up-regulated miRNAs and 14 down-regulated miRNAs at 36 days post-infection (dpi). Among these DEmiRNAs, cfa-miR-193b could participate in the immune response through regulating the target gene cd22 at 24 hpi. Novel_328 could participate in the inflammatory and immune responses through regulating the target gene tgfb1 and tespa1, and enhance the protective effects on the host and inhibit the infection of T. canis at 96 hpi. Moreover, cfa-miR-331 and novel_129 were associated with immune response and self-protection mechanisms at 36 days post-infection (dpi). KEGG pathway analysis showed that 20 pathways were significantly enriched, most of which were related to inflammation response, immune response, and cell differentiation, such as Cell adhesion molecules (CAMs), ECM-receptor interaction, Focal adhesion. These findings suggested that miRNAs in the bone marrow of Beagle dogs play important roles in the pathogenesis of T. canis infection in dogs and provided base data for understanding the interaction between T. gondii and the hosts.