16S RNA sequencing of gut microbiota for study of gestational diabetes

Based on the Tongji-Shuangliu Birth Cohort study, we conducted a nested case-control study among 201 incident GDM cases and 201 matched controls individual matched on age, gestational week, and date of fecal sample collection. Fecal samples were collected during early pregnancy (11.6±2.0weeks), and GDM was diagnosed based on the criteria from the International Association of the Diabetes and Pregnancy Study Groups at 24-28 weeks of pregnancy. Community DNA were isolated from fecal samples and V3-V4 region of 16S rRNA gene amplicon libraries were sequenced on Illumina Miseq platform.