m6a modifications following morphine exposure or Alkbh5 siRNA in primary cortical neuron cultures

m6a modified RNA and unmodified RNA were quantified in primary neuronal cultures following either siRNA knockdown of Alkbh5 or chronic morphine exposure for 3 days. The goal was to identify mRNA that have m6A modifications following one or more treatment conditions. 4 biological replicates were analyzed per treatment group in a two-color experiment. Cy3 labeling represents unmodified RNA and C5 represents RNA with m6A modification. Please note that two channel arrays were used for these samples but the data were analyzed as single-channel so that each sample represents either IP or supernatent and the sample table includes the normalized signal values. Each raw data file is linked to the corresponding IP sample records.