Single-cell transcriptomics of wild-type and cystic fibrosis (F508del/del) mouse uterus

Female cystic fibrosis (CF) patients are confronted with fertility deficiency, experiencing difficulties in getting pregnant. Previous findings using CF mouse models indicate that CF negatively impacts female fertility in mice as it does in humans. Underlying reasons remain elusive. In particular, it has only poorly been studied whether the endometrium, the inner lining of the womb and crucial tissue for embryo implantation, plays a role. Here, we applied single cell transcriptomics of wild-type (F508del ; WT) and CF (F508) mouse uteri from the Cftr mouse model, to get a detailed view on cellular and molecular/pathway differences in CF versus WT endometrium. Five uteri (WT: 3, CF: 2) were assessed in the estrus cycle of the estrous cycle, one CF uteri was sequenced during the diestrus cycle phase.