Raw and compiled IFC data.

(A) Live vs fixed raw data. Length and width measurements used to compile Fig 1D. (B) DCO vs PI percentages. Cell cycle modelling data from triplicate IFC analysis of C9T7 cells stained with DCO or PI as used to compile Fig 2D. (C-E) KINF Run1-3. Raw and processed data from triplicate IFC analysis of unstained mNG:KINF cells analysed with a laser power of 120 mW. Further details are given in the ‘Notes’ section at the bottom of the worksheet. (F) KINF averages. Mean values + standard deviations for data from the three mNG:KINF replicates, used to compile Fig 5B and 5D. (G) DCO. Gate data from triplicate IFC analysis of DCO-stained mNG:KINF cells analysed with a laser power of 3 mW. Run 2 data is presented in S10 Fig. (H) Calculated timings. Compiled mNG:KINF and DCO IFC data used to generate the cell cycle stage durations presented in Table 1 and Fig 7. Further details are given in the ‘Notes’ section at the bottom of the worksheet. (I) Cytokinesis furrowing. Raw data and average values from visual classification of furrows in mNG:KINF cells with spindles or 2CF (3 replicates) used to compile Fig 6A. (J) Flavopiridol. Raw and processed flow cytometry and IFC data from duplicate C9T7 mNG:KINF cultures treated or not with 5 μgml-1 flavopiridol for 12.4 hrs used to compile Fig 7A and 7B. For flow cytometry analysis, cells were fixed in MeOH and stained with PI, and data collected was gated according to DNA content (2C, Int. 4C). For IFC, the mNG:KINF staining pattern was classified using the processing pipeline and gating strategy described above.
(XLSX)