Figure S1 - A Role for the Chemokine RANTES in Regulating CD8 T Cell Responses during Chronic Viral Infection

RANTES deficient P14 CD8 T cells respond similarly to WT P14 cells in a WT environment. WT and RANTES−/− P14 Tg T cells were transferred into C57BL/6 mice and the mice infected with LCMV clone 13 the following day (A). The gating strategy for identifying WT and RANTES−/− P14 cells is shown (B). On days 10–14 p.i., the WT versus RANTES−/− P14 cells were examined for expression of PD-1 (C) and also cytokine production after stimulation with GP33-44 peptide (D and E). Representative FACs plots are shown (D) and MFI of IFNγ as well as the percentage of IFNγ producing cells also making TNFα (E). (EPS)