p53-bound enhancer elements require locally acting transcription factors

Using a massively parallel reporter assay (MPRA), we examined the effect of local sequence context on p53-dependent enhancer activity. 296 putative p53-bound enhancer elements were selected from the hg19 genome assembly and were systematically randomized in 20bp sections from the 5' end. This allowed the investigation into the requirement for local sequence context and transcription factor motifs in the activity of p53 at enhancers.