List of hit compounds identified in the primary screen.

Non-toxic compounds that reduced the HMGB1-induced TNFα production by 2 standard deviation values are listed in order of potency, according to their inhibitory potency for TNFα secretion. The source library of the compounds, their known biological activity and the respective viability values are shown. Viability was measured by the MTT assay. (Abbreviations: MAP kinase: Mitogen-activated protein kinase, U0126∶1,4-diamino-2,3-dicyano-1,4-bis(2-aminophenylthio)butadiene, MEK: mitogen-activated protein kinase kinase, STAT3: Signal transducer and activator of transcription 3, ST057244∶1-[(2E)-3-(3,4,5-trimethoxyphenyl)prop-2-enoyl]piperidin-2-one, Bay 11-7085: (2E)-3-[[4-(1,1-dimethylethyl)pheny?l]sulfonyl]-2-propenenitrile, Bay 11-7082∶3- [(4- methylphenyl)sulfonyl]- (2E)- propenenitrile, ST009819: (2R,3R,13R,14R)-3-(phenylcarbonyl)-17,19-dioxa-4-azapentacyclo[14.2.1.0<2,14>. 0<4,13>.0<7,12>]nonadeca-5,7(12),8,10-tetraen-15-one, MNS: 3,4-methylenedioxy-β-nitrostyrene, IkB: inhibitor of nuclear factor κB kinase, NF-κB: nuclear factor κB, HIV: human immunodeficiency virus, Src: sarcoma tyrosine kinase, Syk: Spleen tyrosine kinase).