Next Generation Sequncimg Facilitates Transcriptional Analysis of ?mak-2, ?mak-1 and Wild Type of Neurospora crassa

By analyzing genome-wide transcriptional responses to deletion of each of two MAP kinase coding genes mak-2 (PR-MAP kinase pathway) and mak-1 (CWI-MAP kinase pathway) in Neurospora crassa during protoperithecium formation, 430 genes co-regulated by MAK-1 and MAK-2 were found, functionally enriched at integral components of membrane and oxidoreductase. Overall design: mRNA profiles of ?mak-1, ?mak-2 and wild type of Neurospora crassa at vegetative growth stage and the initial stage of protoperithecium formation.