Global MEF2 target gene analysis in cardiac and skeletal muscle reveals novel regulation of DUSP6 by p38MAPKMEF2 signaling [ChIP-seq]

Identfification of MEF2A target genes using ChIP-exo in skeletla muscle and primary cardiomyocytes. Identfification of MEF2A target genes using ChIP-exo and RNA-seq in skeletal muscle and primary cardiomyocytes. MEF2 plays a profound role in the regulation of transcription in cardiac and skeletal muscle lineages. To define the overlapping and unique MEF2A genomic targets, we utilized ChIP-exo analysis of cardiomyocytes and skeletal myoblasts. Of the 2783 and 1648 MEF2A binding peaks in skeletal myoblasts and cardiomyocytes, respectively, 294 common binding sites were identified. Genomic targets were compared to differentially expressed genes in RNA-seq analysis of MEF2A depleted myogenic cells.本数据集包含两类实验：其一为在骨骼肌与原代心肌细胞（primary cardiomyocytes）中采用染色质免疫沉淀-外显子测序（ChIP-exo）技术鉴定肌细胞增强因子2A（MEF2A）的靶基因；其二为在上述两种细胞中联合应用ChIP-exo与RNA测序（RNA-seq）技术鉴定MEF2A的靶基因。肌细胞增强因子2（MEF2）在心脏与骨骼肌细胞谱系的转录调控中发挥关键作用。为明确MEF2A基因组靶标的重叠与特异性特征，研究人员对心肌细胞与骨骼肌成肌细胞（skeletal myoblasts）开展了ChIP-exo分析。在骨骼肌成肌细胞与心肌细胞中分别鉴定到2783个和1648个MEF2A结合峰，其中共识别出294个共同结合位点。将上述基因组靶标与MEF2A敲降的肌源性细胞的RNA-seq差异表达基因进行了比对分析。