C. elegans embryonic timecourse in wt and mutant embryos

This series of samples comprises multiple early embryonic time courses for C. elegans. Time courses consisting of 10 time points each for 4 different genotypes are included: wild-type (strain N2 grown on E. coli strain OP50), pie-1(zu154) (progeny of homozygous mutant mothers [Unc] of strain JJ532 grown on E. coli strain OP50), pie-1(zu154);pal-1(RNAi) (progeny of homozygous mutant mothers [Unc] of strain JJ532 grown on E. coli strain HT115 expressing pal-1 hairpin RNA), and mex-3(zu155);skn-1(RNAi) (progeny of homozygous mutant mothers [Dpy] of strain JJ518 grown on E. coli strain HT115 expressing skn-1 hairpin RNA). Embryos were manually staged by morphology at the 4-cell stage and allowed to develop in water for defined amounts of time at 22 degrees C. RNA was amplified as described (Baugh et al. Development, 2003; Baugh et al. Nucleic Acids Research, 2001). This series of samples comprises all replicate data reported by Baugh et al. (Development, 2005). Keywords: time-course本样本系列包含秀丽隐杆线虫（C. elegans）的多组早期胚胎时序数据集。本次数据集涵盖4种不同基因型的胚胎时序样本，每组均包含10个时间点，具体如下：1. 野生型：品系N2，培养于大肠杆菌（E. coli）OP50菌株；2. pie-1(zu154)：品系JJ532纯合突变母本的后代（表现为[Unc]表型），培养于大肠杆菌OP50菌株；3. pie-1(zu154);pal-1(RNA干扰，RNAi)：品系JJ532纯合突变母本的后代（表现为[Unc]表型），培养于表达pal-1发夹RNA的大肠杆菌HT115菌株；4. mex-3(zu155);skn-1(RNA干扰，RNAi)：品系JJ518纯合突变母本的后代（表现为[Dpy]表型），培养于表达skn-1发夹RNA的大肠杆菌HT115菌株。所有胚胎均于4细胞期通过形态学特征进行人工分期，随后置于22℃水中培养至预设时长。RNA扩增方法参照已发表文献（Baugh等，《Development》，2003；Baugh等，《Nucleic Acids Research》，2001）。本样本系列包含Baugh等人于2005年发表在《Development》上的全部重复实验数据。关键词：时间进程（time-course）