High-fat intake reshapes the circadian transcriptome profile and metabolism in murine meibomian glands

Here, we compared the altered transcriptome of MGs in mice fed a balanced diet and a high-fat diet. Then, the effect of metabolic stress generated by HFD on the circadian clock of MGs and its possible underlying mechanisms were explored by bioinformatic analysis and detection of diurnal oscillations of lipid droplet in MGs. We found that increased lipid content in food drastically altered the characteristics of the circadian transcriptome of MGs and produced previously unobserved effects on the transcriptome of MGs. This might provide a pathophysiological basis for explaining how food components affect the physiological function of MGs and bring about certain diseases.