Transcriptional profiling to elucidate processes regulated by 2',3'-cyclic nucleotide monophosphates in Salmonella enterica serovar Typhimurium (S. Typhimurium)

The impact of 2',3'-cyclic nucleotide monophosphates (2',3'-cNMPs) on global gene expression in S. Typhimurium was investigated by heterologous expression of a 2',3'-cyclic nucleotide phosphodiesterase (CNPase), which reduces intracellular 2',3'-cNMP levels. Global transcript levels in S. Typhimurium expressing CNPase were compared to levels in control cells expressing a catalytically inactive variant of the enzyme to identify cellular processes modulated by 2',3'-cNMPs.